Coding

Part:BBa_K2132100:Design

Designed by: Shijie Gu   Group: iGEM16_ShanghaitechChina   (2016-10-14)


HydA,E,F,G together with loxP sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1916
    Illegal NheI site found at 1939
    Illegal NheI site found at 3290
    Illegal NheI site found at 3313
    Illegal NheI site found at 4847
    Illegal NheI site found at 4870
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2865
    Illegal BamHI site found at 5439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 790
    Illegal AgeI site found at 1300
    Illegal AgeI site found at 2971
    Illegal AgeI site found at 4009
    Illegal AgeI site found at 4306
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3742
    Illegal BsaI.rc site found at 3970
    Illegal BsaI.rc site found at 5407
    Illegal SapI.rc site found at 1005
    Illegal SapI.rc site found at 3381
    Illegal SapI.rc site found at 5205


Design Notes

There are many loxP sites.


Source

Clostridium acetobutylicum. However, in our project, it is synthesized.

References